M JAK2
Janus Kinase 2 gene; V617F (Exon 14 mutation)
Chronic myeloproliferative disorders (MPD);
Hydroxurea
Real-time PCR
10 days
5.0 mL (min. 3.0 mL) whole blood, 3.0 mL (min. 1.0 mL) bone marrow EDTA, Sodium Heparin or ACD
Ship at ambient temperature, cool in summer. (Do not allow cool/refrigerated pack to directly contact sample.)
Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimen older than 7 days
V617F mutation detected / not detected
Evaluation of patients with a high index of clinical suspicion for a Philadelphia chromosome-negative (BCR/ABL negative) myeloproliferative disorder (polycythemia vera, essential thrombocythemia, idiopathic myelofibrosis)