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T-Cell TCR Gene Rearrangement by Next Generation Sequencing

Test Code


Test Synonyms

T-cell receptor gamma (TCRγ) gene rearrangement, T cell gene rearrangement


Next Generation Sequencing (NGS)

Turnaround Time

5-7 days

Specimen Requirements

5.0 mL (min. 3.0 mL) whole blood EDTA or Sodium Heparin
3.0 mL (min. 1.0 mL) bone marrow EDTA or Sodium Heparin
5 mm^3 bone marrow core biopsy, fresh tissue or frozen tissue in MPLN RPMI
3.0 mL (min. 2.0 mL) FNA in MPLN RPMI medium
Formalin fixed paraffin embedded tissue

Specimen Stability
Whole blood or bone marrow stable at 18-25°C for 72 hours
Bone marrow biopsy, fresh tissue or FNA in MPLN RPMI stable at 2-8°C for 72 hours
Frozen tissue stable at -20°C indefinitely
Paraffin embedded tissue stable at 18-25°C indefinitely
Storage & Handling

Whole blood, bone marrow, bone marrow biopsy, fresh tissue or FNA, ship in a Styrofoam container with a cool/refrigerated pack (Do not allow the cool pack to directly contact the sample.)
Frozen tissue, ship on dry ice
Paraffin embedded tissue, ship ambient

Causes for Rejection

Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimen older than 7 days

Reference Range

No rearrangement detected (germline)


Molecular characterization of T-cell receptor gene rearrangements and immunoglobulin heavy and light chain gene rearrangement detection is accomplished with reliable and robust tests to identify clonal T-cell and B-cell populations in a variety of cutaneous lymphoproliferative disorders.

While the demonstration of a clonal T/B cell population is not necessarily reflective of a truly pathologic process, positive results interpreted in the context of all laboratory findings can support diagnosis of malignancy. The ability to routinely perform these studies make these highly sensitive and specific tests well suited for the evaluation of these conditions.

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