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Plasma Cell Neoplasms Panel by FISH

Test Code


Test Synonyms

1p-, 1q+, 5,+9, t(11;14), 13q-, +15, 17p-, reflex t(4;14) / t(14;16)


Multiple Myeloma

Reflex testing
F MM will be auto reflexed to F FGFR3 t(4;14) and F IGH MAF t(14;16) when IGH is rearranged with a locus other than BCL1

Fluorescence in situ Hybridization (FISH)

1p32.3 (CDKN2C)/1q21 (CKS1B)
5p15.31 (D5S630,D5S2064)/5q31.2 (EGR1)
CEP 9 (D9Z1)
11q13 (CCND1)/14q32.3 (IGH)
CEP 15 (D15Z4)
13q14.3 (D13S319)/13q34
17p13 (TP53)/CEP17 (D17Z1)

Turnaround Time

3-5 days

Specimen Requirements

2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted

Specimen Stability
Peripheral blood and bone marrow stable at 18-25°C for 72 hours
Storage & Handling

Whole blood and bone marrow, ship ambient

Causes for Rejection

Clotted specimen; Specimens exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells

Reference Range

See report


Cytogenetic studies using chromosome analysis and interphase fluorescence in situ hybridization (FISH) are routine procedures for prognostic stratification of plasma cell myeloma. Several cytogenetic abnormalities such as t(4;14), del(17/17p), t(14;16), nonhyperdiploidy, and gain(1q) were shown to confer poor prognosis. Bone marrow samples used for plasma cell neoplasia testing are sorted for CD138 positive selection cells using magnetic beads. FISH analysis on the CD138 positive selected cells has been shown to increase diagnostic sensitivity. 

In cases with inadequate /insufficient sample, CD138+ enrichment will not be attempted.”

  1. Palumbo A, Herve A, Stefania O, et al. Revised International Staging System for Multiple Myeloma: A Report From International Myeloma Working Group. J Clin Oncol September 2015 33:2863-2869.
  2. Ross F, Herve A, Ameye G, et al. Report from the European Myeloma Network on interphase FISH in multiple myeloma and related disorders. Haematologica August 2017 97:1272-1277.