Acute promyelocytic leukemia (APL);
Fluorescence in situ Hybridization (FISH)
For STAT please call lab
2.0 mL (min. 1.0 mL) peripheral blood in sodium heparin preferred, EDTA accepted
1.0 mL (min. 0.5 mL) bone marrow in sodium heparin preferred, EDTA accepted
5 mm^3 fresh tissue or 3.0 mL (min. 2.0 mL) FNA in MPLN RPMI media
Whole blood and bone marrow, ship ambient.
Fresh tissue or FNA ship in a Styrofoam container with a cool/refrigerated pack (Do not allow cool pack to directly contact sample)
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells; Improper fixative
In a normal cell, a two orange and two green signal pattern is observed. In an abnormal cell containing the t(15;17), one orange, a one green and a two fusion signal pattern is observed.
Acute promyelocytic leukemia (APL) accounts for 10% of adult myeloid leukemia and is frequently associated with a translocation involving the promyelocytic leukemia (PML) gene at 15q22 and the retinoic acid α-receptor (RARα) gene at 17q21 resulting in fusion of the genes PML and RARA. Although all patients with APL have t(15;17) or a variant of this translocation, conventional cytogenetic methods detect these translocations in only 70–96% of patients at diagnosis. FISH can detect this translocation in either interphase or metaphase cells.