Karyotype analysis, cancer cytogenetics
24, 48 and/or 72 hour culture / G-banding
3 mL sodium heparin tube for bone marrow aspirate
5 mm of bone core sample in at least 4 mL tissue culture transport media (RPMI) using 10 mL sterile transport tube
Fixed cytogenetically prepared cells in sterile centrifuge tube with 3:1, Methanol:Acetic Acid. Pellet must be visible for the volume part and the medium part 3:1, Methanol:Acetic Acid.
4 to 25°C during transit, but specimens may be transported on refrigerated gel packs. Do not allow the gel pack to come in contact with the specimen. Do not freeze. Extreme temperatures should be avoided.
Clotted specimen; Specimen exposed to extreme temperature; Anticoagulant toxic to cells; Insufficient number of cells
Chromosome count and morphology consistent with normal karyotype
Bone marrow is the tissue of choice for cytogenetic study of most hematologic conditions because it more accurately reflects what occurs in vivo than other sample types. In hematological malignant diseases, the recognized recurring chromosomal abnormalities often correlate with particular subtypes of leukemia that have characteristic morphological and clinical features, such as response to therapy.
The study of chromosomal abnormalities in leukemia serves two functions. The first of these is to assist in more accurate diagnosis, thereby providing prognostic information and allowing the more rational sections of therapy for a particular patient; and the second is to identify the sites of consistent rearrangements, providing the precise localization required for the isolation and cloning of DNA from these regions.